Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation
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چکیده
منابع مشابه
Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation
Yeast two-hybrid (Y2H) has been successfully used for genome-wide screens to identify protein-protein interactions for several model organisms. Nonetheless, the logistics of pair-wise screening has resulted in a cumbersome and incomplete application of this method to complex genomes. Here, we develop a modification of Y2H that eliminates the requirement for pair-wise screening. This is accompli...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2007
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkm894